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KMID : 1094719970020020090
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 2 p.90 ~ p.93
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Continuous production of fructose-syrups from inulin by immobilized inulinase from recombinantSaccharomyces cerevisiae
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Kim Byung-Woo
Kim Han-Woo
Nam Soo-Wan
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Abstract
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Recombinant exoinulinase was partially purified from the culture supernatant ofS. cerevisiae by (NH4)2SO4 precipitation and PEG treatment. The purified inulinase was immobilized onto Amino-cellulofine with glutaraldehyde as a cross-linking agent. Immobilization yield based on the enzyme activity was about 15%. Optimal pH and temperature of immobilized enzyme were found to be 5.0 and 60°C, respectively. The enzyme activity was stably maintained in the pH ranges of 4.5 to 6.0 at 60°C. 100% of enzyme activity was observed even after incubation for 24 hr at 60°C. In the operation of a packed-bed reactor containing 412 U inulinase, dahalia inulin of 7.5%(w/v) concentration was completely hydrolyzed at flow rate of 2.0 mL/min at 60°C, resulting in a volumetric productivity of 693 g-reducing sugars/L/h. Under the reaction conditions of 1.0 mL/min flow rate with 2.5% inulin at 60°C, the reactor was successfully operated over 30 days without loss of inulinase activity.
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KEYWORD
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Immobilization, recombinant inulinase, fructose-syrup, packed-bed reactor
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